Preparation of Acellular Myocardial Scaffolds with Well-preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

Wang, B., Williams, L. N., Curry, A. L., & Liao, J. (2014). Preparation of Acellular Myocardial Scaffolds with Well-preserved Cardiomyocyte Lacunae, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct. Cardiac Tissue Engineering Methods and Protocols. Springer Science.

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit to myocardial infarction (MI) treatment. Here we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulations for facilitating cardiac construct development.